Table 2.
Use and characteristics of primary antibodies
| Primary antibody to | Raised in species | Code | Dilution | Protein conc. original | Source | Characterization, reference, or test | Immunogen |
|---|---|---|---|---|---|---|---|
| Calbindin | Rabbit | CB-38 | 1 : 1000 | Swant, Bellizona, Switzerland | Labeling pattern in the rat as published with other antibodies | Rat recombinant calbindin | |
| Calretinin (CR) | Goat | CG1 | 1 : 1000 | Swant, Bellizona, Switzerland | Schwaller et al. (1999), western blot, brain, and cell lines expressing recombinant CR | Recombinant human calretinin | |
| COUP-TFII | Mouse | PP-H7147-00, 2ZH7147H | 1 : 1000 | 1 mg/mL | Perseus Proteomics, Inc./R&D Systems | Qin et al. (2007), tested in gene deleted tissue | Recombinant human COUP-TFII |
| NPNFP, non-phosphorylated neurofilament protein | Mouse | SMI-311R | 1 : 1000 | Convance/Cambridge Biosciences | Mixture of several monoclonal antibodies recognizing NF-H and NF-M subunits. Originally developed by Sternberger Monoclonals, Inc. | Brain | |
| Parvalbumin | Goat | PVG 214 | 1 : 1000 | Swant, Bellizona, Switzerland | Labeling pattern in the rat as published with other antibodies | Rat muscle parvalbumin | |
| Pro-CCKa | Rabbit | 1 : 2000 | A. Varro, Liverpool University | Morino et al. (1994) | Residues 107–115 of rat pro-CCK | ||
| Reelin | Mouse | MAB5366 | 1 : 50000 | 1 mg/mL | Chemicon | de Bergeyck et al. (1998), immunoblot | Recombinant mouse reelin residues 40–189 |
| Somatostatin | Rat | MAB354 | 1 : 200 | Chemicon | Labeling pattern in the rat as published with other antibodies | Synthetic cyclic somatostatin 1–14 |
aThis antibody labels the Golgi apparatus in the soma where pro-CCK is processed and packaged for transport, and for simplicity the signal obtained is described in the text as CCK.