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. 2016 Feb 26;7:206. doi: 10.3389/fpls.2016.00206

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Copyright © 2016 Hosseini, Hajirezaei, Seiler, Sreenivasulu and von Wirén.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of K supply on K concentrations and gene expression of K transporters in flag leaves of barley during terminal drought stress. (A) K concentrations, (B) relative HvHAK4 mRNA levels, and (C) relative HvAKT2 mRNA levels in flag leaves of the lines HPR and LPR. Plants were pre-cultured under sufficient water supply (control) or water limitation (stress) and under low or high K supply. Flag leaves from 12 weeks-old plants were harvested 12 days after imposing drought stress. Bars indicate mean ± SD. Different letters denote significant differences according to three-way ANOVA and Tukey’s test (p < 0.05; n = 6). Ubi E2-17 (ubiquitin-conjugating enzyme) was used as reference gene.