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. 2015 Dec 23;107(2):133–139. doi: 10.1111/cas.12848

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Daple regulates Wnt5a‐induced Rac/JNK activation and laminin γ2 expression in gastric cancer cells. (a) Daple knockdown inhibited Wnt5a‐induced laminin γ2 expression, Rac activation and JNK phosphorylation. Western blot analysis of total cell lysates from MKN45 cells transfected with the indicated combinations of plasmids (control or Wnt5a) and siRNA (control or Daple siRNA). For Rac activation analysis (lower two panels), GTP‐bound Rac1 was pulled down with GST‐PBD and precipitated samples were probed with Rac1 antibody. (b,c) Wnt5a‐induced migration or invasion was attenuated by Daple knockdown. Transwell migration (b) or invasion (c) assays of MKN45 cells transfected with the indicated combinations of plasmids. Migrated cell numbers were expressed as the relative migration divided by that of Wnt5a (−) cells. The results represent the means ± SE. *P < 0.05.