Figure 3.
Daple knockdown attenuates migration and invasion of high‐Wnt5a‐expressing KKLS cells. (a) Western blot of total cell lysates of control and Daple knockdown KKLS cells using three different shRNA (shDaple#1‐3). (b) LAMC2 expression in control and Daple knockdown KKLS cells quantified by real‐time RT‐PCR, normalized to 18S ribosomal RNA (18S) expression. The results represent the means ± SE. *P < 0.05 compared with control shRNA. NS, not significant. (c,d) Migration (c) and invasion (d) assays of control shRNA or Daple shRNA#2‐expressing KKLS cells. The number of migrated cells was expressed as relative migration (%) divided by that of control cells. The results represent the means ± SE. (e) Daple knockdown had no effect on KKLS cell proliferation. 1 × 105 KKLS cells stably expressing control (closed square) or Daple (open square) shRNA were cultured for 3 days; cell numbers were counted each day.