Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 2;107(2):116–122. doi: 10.1111/cas.12853

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Immunohistochemical staining of natural killer group 2 member D (NKG2D) ligands in human extrahepatic cholangiocarcinoma tissues. Representative images of immunohistochemical staining of specimens are shown. Resected extrahepatic cholangiocarcinoma specimens were stained using standard streptavidin–biotin–peroxidase complex methods and 3,3′‐diaminobenzidine tetrahydrochloride and counterstained with Mayer's hematoxylin. The expression of NKG2D ligands was evaluated according to the staining intensity observed and scored as follows: 0, negative; 1, weak expression; and 2, strong expression. Cases with scores of 0 and 1 were defined as the low‐expression group, and cases with scores of 2 were defined as the high‐expression group. MHC class I chain‐related proteins A and B (MICA/B) (a), unique long 16 binding protein 1 (ULBP1) (b), and ULBP2/5/6 (c) low/high expression.