Skip to main content
. 2016 Jan 26;107(2):140–148. doi: 10.1111/cas.12849

Figure 1.

Figure 1

EW‐7197 inhibits the colony‐forming capacity of murine CML‐LICs in vitro. (a,b) Colony‐forming capacity of freshly isolated GFP/BCR‐ABL1+ cKit+Lineage−Sca1+ (KLS) CML multipotent progenitors (CML‐MPPs) that were cocultured on OP‐9 stromal cells under hypoxic conditions (3% O2) and treated for 3 days with either DMSO (control) or the indicated concentrations of EW‐7197 (a), or for 3 days with DMSO or EW‐7197, without or with 1 μM IM (b). Colony‐forming capacity was determined by culture in methylcellulose. Data shown are the mean colony number ± SD (n = 3). (c,d) Colony‐forming capacity of freshly isolated T315I‐BCR‐ABL1‐GFP + KLS CML‐MPPs that were treated for 3 days with either DMSO (vehicle control), 1 μM imatinib mesylate (IM), or 5 μM EW‐7197 (c), or for 3 days with DMSO or EW‐7197, with the addition (or not; DMSO) at 24 h post‐EW‐7197 treatment of 1 μM ponatinib for 48 h (d). Results were analyzed as for (a,b). NS, not significant.