Table 1. Predicted molecular weights and mobile fractions for Venus, Venus-LC3B, and Venus-LC3B mutants based on the FRAP diffusion measurements in live HEK 293 cells under basal conditions.

Construct	Monomer MW (kDa)	FRAP predicted MW (kDa)*	FRAP mobile fraction (%)
Venus	27	N/A	100 ± 1 (34)
Venus-LC3B	45	500 ± 200 (30)	99 ± 1 (30)
Venus-LC3B (F52A/L53A)	45	1200 ± 400 (30)	100 ± 1 (30)
Venus-LC3B (R70A)	45	130 ± 50 (30)	102 ± 2 (30)

*Calculated assuming diffusing species has a spherical shape; mean ± 95% CI (N, # cells).