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(a) COMSOL model of A350 (upper left port) and A488 (right port) diffusion gradient after 2 hours. (b) A350, (c) A488 (bottom) experimentally measured diffusion normalized to maximum fluorescence across 3 hours confirming formation of gradient within 5 minutes and presence of gradient after 3 hours. Both fluorescent dyes were added and detected from the same device to show the detection of two competing gradients. F.I. : Fluorescence Intensity
