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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Dev Biol. 2016 Jan 7;410(2):202–212. doi: 10.1016/j.ydbio.2015.12.008

Figure 3. Zfrp8 is required for proper FMRP localization.

Figure 3

A–A”, E–E’. In wild-type (w118) ovarioles FMRP is seen as puncta throughout the cytoplasm and at low levels in nurse cell nuclei of stage 8 egg chambers at an average of 9.76 puncta per nucleus (N=80), while Zfrp8 is uniformly distributed in the cytoplasm and nuclei. B–B”, F–F’. In Fmr1Δ50M/Df(3R)Exel6265 ovarioles FMRP protein is absent as expected both in the cytoplasm and in nuclei (N=81), while Zfrp8 expression and distribution is not significantly changed. C–C”, G–G’. Control nos-Gal4/+ ovarioles appear similar to wild-type, FMRP localizes to cytoplasmic foci;. D–D”, H–H’. Within Zfrp8 KD egg chambers FMRP localization to cytoplasmic puncta is disrupted and the protein is more uniformly distributed throughout the cytoplasm. Anti-Zfrp8 (green), anti-FMRP (red), DNA (blue). Confocal sections are shown; scale bar: 10 uM.