Figure 1. Effects of zymosan on the expression of IL-6, IL-8, and MCP-1 in HCFs.

A: Serum-deprived cells were incubated for 24h in the absence or presence of zymosan (600 µg/mL), after which the release of IL-6, IL-8, and MCP-1 into the culture medium was determined with ELISAs. B: Serum-deprived cells were incubated for 12h in the absence or presence of zymosan (600 µg/mL), after which total RNA was isolated from the cells and subjected to RT-qPCR analysis of IL-6, IL-8, and MCP-1 mRNA abundance. All data are means±SD of triplicates from experiments that were repeated a total of three times with similar results. ^aP<0.05 (Student's t-test) versus the corresponding value for cells cultured without zymosan.