Figure 2. Concentration-dependent inhibition by triptolide of zymosan-induced IL-6, IL-8, and MCP-1 release from HCFs.

Serum-deprived cells were incubated first for 1h in the absence or presence (1 to 30 nmol/L) of triptolide and then for 24h in the additional absence (open circles) or presence (closed circles) of zymosan (600 µg/mL), after which the release of IL-6 (A), IL-8 (B), and MCP-1 (C) into the culture medium was determined with ELISAs. Data are means±SD of triplicates from experiments that were repeated a total of three times with similar results. ^aP<0.05 versus the corresponding value for cells cultured without triptolide (Tukey-Kramer test).