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. 2016 Jan 18;9(1):9–14. doi: 10.18240/ijo.2016.01.02

Figure 4. Effects of triptolide on the zymosan-induced up-regulation of IL-6, IL-8, and MCP-1 mRNA abundance in HCFs.

Figure 4

Serum-deprived cells were incubated first with or without 30 nmol/L triptolide (trip) for 1h and then in the additional absence or presence of zymosan (600 µg/mL) for 12h, after which total RNA was isolated from the cells and subjected to RT-qPCR analysis of IL-6 (A), IL-8 (B), and MCP-1 (C) mRNA abundance. Data are means±SD from three separate experiments. aP<0.001 (Tukey-Kramer test) versus the corresponding value for cells cultured without zymosan; bP<0.01 (Tukey-Kramer test) versus the corresponding value for cells cultured without triptolide.