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. 2016 Feb 26;7:208. doi: 10.3389/fmicb.2016.00208

FIGURE 2.

FIGURE 2

Variation of ABgp46 tertiary and secondary structure with pH and temperature. (A) Correlation between fluorescence spectra and conformational changes deduced by plotting Parameter A as a function of the same pH and temperature ranges, for tertiary structure analysis. (B) Far-UV Circular dichroism thermal denaturation profiles (from 20 to 70°C, with heating rates of 2°C/min), measured at 222 nm, and at different pH values (pH 4.0–10.0) for secondary structure analysis.