Table 2.
Combinatorial antibacterial activity of ABgp46 with outer membrane permeabilizers under different concentrations against Escherichia coli O157:H7.
| pH | OMPs | Molarity (mM) | PBS/OMPs | ABgp46/OMPs | ABgp46/OMPs |
|---|---|---|---|---|---|
| + 5 mM MgCI2 | |||||
| 7.0 ± 0.1 | EDTA | 0.50 | 0.27 ± 0.11 | 0.32 ± 0.15 | |
| Citric | 0.36 | 0.21 ± 0.14 | 0.86 ± 0.28 | ||
| Malic | 0.60 | 0.24 ± 0.11 | 0.53 ± 0.28 | ||
| Lactic | 1.20 | 0.24 ± 0.14 | 0.55 ± 0.31 | ||
| Benzoic | 1.20 | 0.26 ± 0.15 | 0.49 ± 0.34 | ||
| Acetic | 1.20 | 0.25 ± 0.14 | 0.53 ± 0.30 | ||
| 5.5 ± 0.1 | Citric | 1.50 | 0.20 ± 0.13 | 1.45 ± 0.25 | |
| Malic | 3.30 | 0.17 ± 0.14 | 1.09 ± 0.29 | ||
| Lactic | 3.45 | 0.31 ± 0.07 | 0.95 ± 0.14 | - | |
| Benzoic | 3.55 | 0.27 ± 0.13 | 0.44 ± 0.33 | ||
| Acetic | 4.35 | 0.26 ± 0.12 | 0.21 ± 0.22 | ||
| 4.0 ± 0.1 | Citric | 3.65 | 0.28 ± 0.06 | 2.78 ± 0.37 | 0.15 ± 0.04 |
| Malic | 4.55 | 0.24 ± 0.18 | 2.08 ± 0.32 | 0.10 ± 0.03 | |
| Lactic | 8.00 | 0.27 ± 0.18 | 0.94 ± 0.28 | 0.15 ± 0.04 | |
| Benzoic | 10.00 | 0.57 ± 0.58 | 1.11 ± 0.27 | 0.02 ± 0.03 | |
| Acetic | 20.00 | 0.22 ± 0.08 | 0.42 ± 0.29 | 0.11 ± 0.10 |
For each specific pH, different concentrations of EDTA, citric, malic, lactic, benzoic, and acetic acid (given in mM) are used in combination with 2 μM of ABgp46 to reduce E. coli O157:H7 cells in presence or absence of 5 mM of MgCl2. Averages ± standard deviations for all experiments are given for n = 4 repeats.Marked in bold are ≥1 log reduction units observed.