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. 2016 Feb 23;4:e1734. doi: 10.7717/peerj.1734

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©2016 Ferreira et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Cluster of hrp genes in the Xac genome, highlighting the mutation of the hrpB4 gene induced by insertion of the transposition cassette EZ-Tn5 <Kan >. The insertion was made at position 147 in the gene. (B) Structural composition of the corresponding apparatus to the type III secretion system encoded by hrp genes. The HrpB4 protein is highlighted in red. (C) Phenotype virulence alteration of the mutant ΔhrpB4 (complete absence of symptoms) compared to the wild-type strain (WT) after 12 days of infection in Citrus sinensis plants.