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. 2016 Feb 16;4:e1706. doi: 10.7717/peerj.1706

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©2016 Cao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Langendorff hearts were treated with 5-HD (A) or IPo (B) after I/R insult. Total mitochondrial protein samples were separated using linear IPG strips (24 cm, pH 5–8), and stained with silver nitrate. (C) Protein spots with differential parts per million (PPM) value were annotated with numbers in an image synthesized by PDQuest software. Protein in spot 3002 (detected as ATPA by MASCOT peptide mass fingerprint (PMF) and indicated with a black arrow) was detected as a differential protein by PDQuest. (D) Enlarged 2DE gel images and 3D view showing the 5-HD- and IPo-dependent protein expression changes of ATPA.