Table 1. Information list of differential proteins.
Comparison | Protein name | Abbreviation | Calculated PI | Calculated Mr | SSPa | NCBI no. | AA% coverageb | Scorec | Fold changed | P valuee |
---|---|---|---|---|---|---|---|---|---|---|
Nor vs I/R | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial | SDHA | 6.75 | 72,596 | 5,805 | gi|18426858 | 23% | 326 | 2.40 ± 0.24 | 0.002 |
I/R vs IPo | NADH dehydrogenase (ubiquinone) flavoprotein 1, isoform CRA_b | NDUFV1 | 5.89 | 36,844 | 1,507 | gi|149061921 | 32% | 176 | 2.3 ± 0.53 | 0.005 |
Dihydrolipoyl dehydrogenase, mitochondrial precursor | DLD | 7.96 | 54,574 | 2,706 | gi|40786469 | 14% | 62 | 2.58 ± 0.49 | 0.008 | |
IPo vs 5-HD | Dihydrolipoyl dehydrogenase, mitochondrial precursor | DLD | 7.96 | 54,574 | 1,609 | gi|40786469 | 19% | 156 | −2.96 ± 0.83 | 0.003 |
rCG44606 | rCG44606 | 9.13 | 40,522 | 2,107 | gi|149059002 | 21% | 134 | −2.72 ± 0.98 | 0.003 | |
ATP synthase subunit alpha, mitochondrial | ATPA | 7.03 | 25,639 | 3,002 | gi|57029 | 44% | 185 | −2.02 ± 0.29 | 0.003 | |
MICOS complex subunit Mic60 (mitochondrial inner membrane protein) | IMMT | 5.57 | 67,477 | 6,804 | gi|77917546 | 22% | 253 | 2.15 ± 0.82 | 0.012 | |
Stress-70 protein, mitochondrial (GRP-75) | GRP75 | 5.87 | 73,984 | 7,803 | gi|1000439 | 15% | 194 | 2.54 ± 0.84 | 0.001 |
Notes.
Protein spot numbers given by PDQuest.
Differential proteins’ amino acid coverage rate in reference proteins in NCBInr.
By MALDI-TOF MS analysis.
Expression change (more than ±200%), n = 4. For the ratio of Fold change of A vs B, “+” means B is overexpressed, “−” means B is down-regulated.
Statistics of expression level expressed as parts per million (PPM) with unpaired t test, n = 4.