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Evaluation of new matrix formation. Masson’s Trichrome staining of subcutaneous implants in rats showing deposition of new collagen matrix at day 5 (top row). By day 10 (bottom row), significant degradation of peptide matrix is observed, with new collagen matrix deposited where the implant material existed previously. Masson’s Trichrome stain indicates muscle fibers in red, collagen in blue, cell cytoplasm in pink, and cell nuclei in dark brown. Scale bar 500 μm.
