Table 3. Effect of different donors on in vitro development of bovine embryos generated by OPU-IVF.
Group | Donor | Age | Parity | No. of oocytes | No. (%) of cleaved embryos | No. (%) of blastocysts | ||
Low embryo production | A | 16 | 9 | 74 | 11 | (16.2 ± 7.6) a | 2 | (2.7 ± 2.4) a |
B | 10 | 2 | 73 | 19 | (31.5 ± 10.2) ab | 4 | (5.5 ± 2.7) ab | |
C | 10 | 2 | 83 | 28 | (38.6 ± 3.7) ab | 6 | (7.2 ± 0.6) ab | |
D | 9 | 1 | 96 | 32 | (33.3 ± 6.4) ab | 13 | (13.5 ± 2.1) ab | |
Conventional | E | 13 | 2 | 52 | 18 | (34.6 ± 10.7) ab | 13 | (25.0 ± 9.4) ab |
F | 13 | 1 | 49 | 24 | (49.0 ± 7.4) ab | 14 | (28.6 ± 6.5) ab | |
G | 13 | 3 | 45 | 36 | (80.0 ± 5.0) b | 16 | (35.6 ± 9.8) b | |
H | 11 | 4 | 126 | 93 | (73.8 ± 16.1) b | 45 | (35.7 ± 7.9) b | |
I | 14 | 8 | 76 | 50 | (65.8 ± 5.6) b | 33 | (43.4 ± 9.2) b | |
J | 12 | 3 | 70 | 46 | (65.7 ± 8.7) ab | 32 | (45.7 ± 4.4) b | |
K | 15 | 6 | 64 | 49 | (76.6 ± 18.0) b | 32 | (50.0 ± 12.7) b |
Values with superscript letters are statistically significant (P < 0.05). The number of replicates was five. Cleavage and blastocyst rates were evaluated at 72 h and 192 h. Low embryo production differed by ≤ 20% in terms of blastocyst rate.