Fig. 2.

Comparison of the effects of H89 and U0126 on estradiol (0.01 nM) suppression of GnRH-induced LH secretion from cultured bovine AP cells. The final concentrations of H89, U01226 and GnRH were 5000 nM, 1000 nM and 1 nM, respectively. LH concentrations in control cells (cultured in medium alone) were averaged, and the mean LH concentrations of treated groups were expressed as percentages of the average control value. a vs. b: significant difference (P < 0.05).