Table 1. Development of cloned embryos derived from donor cells treated with 5-aza-dC or 5-methyl-dCTP.
| Group | No. embryos (Rep.) | No. embryos fused (% ± SEM) |
No. embryos cleaved (% ± SEM) * |
No. blastocysts (% ± SEM) * |
Total cell numbers of blastocysts (mean ± SEM) & |
| IVF | 224 (5) | — | 175 (78.07 ± 2.22) abc | 52 (23.28 ± 0.88) a | 39 ± 3 (n = 52) |
| Control | 279 (5) | 222 (79.71 ± 1.99) a | 201 (90.31 ± 1.44) d | 48 (21.43 ± 1.20) ab | 37 ± 2 (n = 47) |
| Aza-10 | 278 (5) | 192 (69.46 ± 1.37) b | 153 (79.54 ± 1.35) ace | 36 (18.87 ± 0.72) bc | 35 ± 2 (n = 36) |
| Methyl-1 | 297 (5) | 216 (72.64 ± 3.47) b | 181 (83.50 ± 1.00) e | 40 (18.95 ± 1.12) bc | 35 ± 2 (n = 39) |
| Methyl-2 | 312 (5) | 216 (69.10 ± 1.87) b | 174 (80.79 ± 1.17) ae | 38 (17.63 ± 0.79) c | 36 ± 2 (n = 36) |
| Methyl-5 | 320 (5) | 193 (60.47 ± 2.30) c | 146 (75.77 ± 2.47) c | 28 (14.70 ± 1.17) d | 34 ± 3 (n = 28) |
* Cleavage and blastocyst rates of cloned embryos were adjusted for fusion rates. & Blastocyst cell numbers of less than 16 were not included. a–c Values in the same column with different superscripts differ significantly (P < 0.05).