Fig. 5.

In situ hybridization of Rpf1 and immunohistochemistry for PROP1. In situ hybridization with DIG-labeled anti-sense (A) and sense (B) probes for Rpf1 was performed using the cephalic part at E13.5. A merged image of immunohistochemistry for PROP1 (red) and nuclear staining with 4, 6’-diamidino-2-phenylindole dihydrochloride (DAPI, blue; Molecular Probes, Vector Laboratories, Burlingame, CA, USA) is shown (C). RP, Rathke’s pouch; DE, diencephalon; RT, rostral tip; HT, hypothalamus. Bar 100 µm.