Table 3. Reporter assay of transcription factors for Prop1 (–2993/+21).
| A | B | C | D | Function | Ref. | ||
| SOX2 only | Factor only | SOX2+Factor | C/A | ||||
| SOX2-dependent stimulation | |||||||
| FOXJ1 | 9.9 ± 1.6** | 0.7 ± 0.0** | 15.6 ± 1.3** | 1.6 | Ependymal cell/astrocyte differentiation | [40] | |
| HES1 | 9.0 ± 1.2** | 0.8 ± 0.1 | 21.3 ± 1.9** | 2.4 | Maintain stemness of the stem cell | [41, 42] | |
| HEY1 | 7.3 ± 1.0** | 1.3 ± 0.2 | 28.0 ± 2.9** | 3.8 | Maintain stemness of the stem cell | [41, 42] | |
| HEY2 | 7.3 ± 1.0** | 0.8 ± 0.1 | 15.7 ± 1.1** | 2.2 | Maintain stemness of the stem cell | [41, 42] | |
| SOX2-dependent suppression | |||||||
| KLF6 | 9.9 ± 1.6** | 1.2 ± 0.2 | 7.3 ± 1.3 | 0.7 | Regulator of Prrx2 in the pituitary | [43] | |
| MSX1 | 8.2 ± 0.9** | 0.9 ± 0.2 | 3.7 ± 0.6** | 0.5 | Pituitary organogenesis | [44] | |
| RUNX1 | 7.3 ± 1.0** | 0.8 ± 0.1 | 2.8 ± 0.4** | 0.4 | Hematopoietic/hair follicle stem cells | [45, 46] | |
| TEAD2 | 9.9 ± 1.6** | 1.3 ± 0.4 | 3.1 ± 0.2** | 0.3 | Vessel/neural tube/heart organogenesis | [47] | |
| YBX2 | 7.6 ± 0.5** | 1.4 ± 0.2** | 2.8 ± 0.5** | 0.4 | Stability of germ cell mRNAs | [48] | |
| ZFP36L1 | 7.3 ± 1.0** | 1.1 ± 0.1 | 1.8 ± 0.1** | 0.2 | Vessel/neural tube/heart organogenesis | [49] | |
| SOX2-independent regulation | |||||||
| MSX2 | 6.6 ± 1.1** | 7.7 ± 1.0** | 7.5 ± 2.0 | 1.1 | Cell survival/apoptosis | [50, 51] | |
| PAX6 | 7.3 ± 1.0** | 5.8 ± 0.1** | 7.9 ± 1.2 | 1.1 | Early embryonic pituitary factor | [52] | |
| PIT1 | 9.5 ± 1.9** | 4.8 ± 0.5** | 13.8 ± 1.7 | 1.5 | Generate GH-, PRL- and TSH- cells | [53] | |
| PITX1 | 8.3 ± 0.8** | 9.6 ± 2.7** | 13.0 ± 0.6** | 1.6 | Pan-pituitary activator | [54] | |
| PITX2 | 8.3 ± 0.8** | 16.6 ± 4.3** | 14.4 ± 1.5** | 1.7 | Pituitary formation/cell specification | [55] | |
| RPF1 | 9.9 ± 0.6** | 3.6 ± 0.3** | 13.5 ± 1.8** | 1.4 | Retina/pituitary transcription factor | [13, 56] | |
| SOX8 | 7.6 ± 0.5** | 0.4 ± 0.1** | 6.3 ± 1.1 | 0.8 | Organogenesis | [57] | |
| SOX11 | 9.3 ± 0.4** | 9.2 ± 0.9** | 7.2 ± 1.2 | 0.8 | Neurogenesis and targets TEAD2 | [58, 59] | |
All values are expressed as means ± SD of quadruplicate transfections from two to three independent experiments with reproducible data. Representative data are shown. Statistical analyses for Table 3 were performed as follows. Column A: significance between the values with and without SOX2. Column B: significance between the values with and without factors. Column C: significance between the values with SOX2 + factor and with SOX2 only. ** P < 0.01.