. 2015 Dec 6;62(1):93–102. doi: 10.1262/jrd.2015-092

Table 4. Reporter assay of transcription factors for serially truncated reporter vectors of the Prop1 5’-upstream region.

Reporter vector	Prop1	Prop1	Prop1	Prop1	Prop1	Prop1	Basic
Reporter vector	(–2993/+21)	(–1840/+21)	(–1270/+21)	(–771/+21)	(–443/+21)	(–154/+21)	vector^b)
SOX2	9.4 ± 1.4^**	2.8 ± 0.3^**	1.7 ± 0.5	1.0 ± 0.2	0.7 ± 0.2	0.6 ± 0.1^**	1.0 ± 0.2

MSX2	7.3 ± 0.7^**	3.2 ± 0.5^**	3.1 ± 0.3^**	2.3 ± 0.3^**	1.8 ± 0.3^**	1.1 ± 0.2	1.0 ± 0.1
PAX6	6.1 ± 0.8^**	3.4 ± 0.4^**	2.7 ± 0.2^**	1.3 ± 0.3	0.9 ± 0.1	0.8 ± 0.2	1.0 ± 0.1
PIT1	3.0 ± 0.3^**	1.6 ± 0.2^**	0.8 ± 0.1^**	0.7 ± 0.3	0.8 ± 0.1	1.0 ± 0.1	1.0 ± 0.0
PITX1	6.9 ± 2.2	3.4 ± 0.7	2.1 ± 0.2^**	1.2 ± 0.1	0.9 ± 0.1	0.7 ± 0.2	1.0 ± 0.1
PITX2	17.9 ± 3.7^**	6.7 ± 3.0	5.6 ± 0.9^**	2.6 ± 0.8	2.0 ± 0.4^**	1.0 ± 0.2	1.0 ± 0.1
RPF1	2.9 ± 0.3^**	1.5 ± 0.1^**	1.3 ± 0.2	0.9 ± 0.1	1.1 ± 0.2	0.6 ± 0.1^**	1.0 ± 0.1
SOX8	0.5 ± 0.3	1.0 ± 0.1	0.5 ± 0.1^**	0.5 ± 0.0^**	0.8 ± 0.2	1.0 ± 0.1	1.0 ± 0.2
SOX11	4.4 ± 1.5	1.2 ± 0.2	1.2 ± 0.4	1.1 ± 0.1	1.8 ± 0.2^**	0.6 ± 0.1^**	1.0 ± 0.1
Control^a)	1.0 ± 0.1	1.0 ± 0.0	1.0 ± 0.1	1.0 ± 0.1	1.0 ± 0.1	1.0 ± 0.1	1.0 ± 0.1

All values are expressed in means ± SD of quadruplicate reporter assay using the CHO cell line in two to three independent experiments with reproducible data. Representative data are shown. The statistical significance between the values with and without factors was determined by Student’s t-test. ** P < 0.01. ^a) The control was assayed with the pcDNA3.1 vector. ^b) The basic vector was pSEAP2-Basic.