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. Author manuscript; available in PMC: 2016 Feb 26.
Published in final edited form as: New Phytol. 2015 Jun 23;208(4):1157–1168. doi: 10.1111/nph.13528

Fig. 5.

Fig. 5

S349 and S351 are indispensable for HopZ1a to suppress stomatal defense. Leaf discs of 4-wk-old transgenic Arabidopsis thaliana zar1-1 plants expressing wild-type or mutant HopZ1a were incubated with 10 μM flg22. Two hours after flg22 treatment, stomata on the lower epidermis were observed under a microscope. (a) Micrographs of stomata were used to measure the stomatal aperture, which is expressed as the ratio of width over length. (b) The average stomatal aperture and SE (as error bars). Arabidopsis thaliana zar1-1 expressing HopZ1a(C216A) was used as a negative control. Data for which differences were statistically significant (two-tailed t-test): *, P < 0.05. The experiment was repeated twice with similar results.