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. 2015 Dec 30;18(1):39–46. doi: 10.1515/bjmg-2015-0004

Table 1.

Primers and TaqMan® (Applied Biosystems) probe sequences used in quantitative real-time polymerase chain reaction.

Gene Forward Primer Sequences (5′>3′) Reverse Primer Sequences (5′>3′)
NRF1 GGC ACT GTC TCA CTT ATC CAG GT T CAG CCA CGG CAG AAT AAT TCA
TFAM AAG ATT CCA AGA AGC TAA GGG TGA CAG AGT CAG ACA GAT TTT TTC CAG TTT
MT-CO1 GAG CTG CTG TTC GGT GTC C TGC CAG TGG TAG AGA TGG TTG
HPRT1 TGG ACT AAT TAT GGA CAG GAC TGA AC GCA CAC AGA GGG CTA CAA TGTG
Gene Probe Sequences (5′>3′)
NRF1 FAM-ACC ACG GTC ACC GTT GCC CAA-BHQ1
TFAM FAM-CAC CGC AGG AAA AGC TGA AGA CTG TAA AG-BHQ1
MT-CO1 FAM-CAA TAC CGC AAC CGC ATT GCC AT-BHQ1
HPRT1 FAM-CTC CCA TCT CCT TCA TCA CAT CTC GAG C-BHQ1