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. Author manuscript; available in PMC: 2016 Feb 26.
Published in final edited form as: J AIDS Clin Res. 2016 Jan 31;7(1):540. doi: 10.4172/2155-6113.1000540

Figure 1.

Figure 1

Schematic and images of Dual Path CARD cartridge for screening and confirmatory analysis of HIV infection. (A) Top view of schematic indicating the positions of the sample swab input ports, the types of reagent wells, and lateral flow strip adapters. (B) Top view image of the injection molded CARD cartridge. Flow of sample is initiated once the sample is introduced in the Sample Reservoir. Twenty µl of the sample is pumped through the various diaphragm pumps and channels to the Lateral Flow Strip Adapter compartment where the lateral flow antibody assay occurs. Simultaneously, the remaining 200 µl of the sample reacts with listed reagents which are loaded to Reagent Reservoir and pumped to Sample Reservoir or Bead Processing Reservoir. Beads are captured at Bead Processing Diaphragm and eluent is pumped to Elution Reservoir. Eluent and LAMP master mix from Master Mix Reservoir are pumped to LAMP Amplification Tube for LAMP reaction.