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. 2016 Feb 22;11(2):e0148905. doi: 10.1371/journal.pone.0148905

Fig 3. PRMT5 knockdown reduces association of p65 with the CXCL11 promoter.

Fig 3

The location of major regulatory elements and primer binding sites used for the chromatin immunoprecipitation (ChIP) assay of the CXCL11 promoter are illustrated in (A). IFN-γ response elements include the gamma interferon activation site (GAS) and interferon-sensitive response element (ISRE). The major TNF responsive element is the NF-κB binding site. ChIP assays were performed to assess p65 binding with the proximal promoter encompassing the κB binding site(s) of CXCL11 (B), CXCL10 (C), and CCL2 (D) in PRMT5-intact or -depleted EC stimulated with TNF plus IFN-γ for 30 minutes. *, p < 0.05; **, p < 0.01; error bars represent S.E. (n = 3).