Fig 4. Mutation of Arg¹⁷⁴ to lysine abrogates p65 association with the CXCL11 promoter.

Endogenous p65 was depleted with siRNA targeting the 3´ UTR of p65 in EC. p65 was reconstituted in these cells by transfecting cDNA encoding FLAG-tagged wild type p65 or the FLAG-tagged Arg¹⁷⁴Lys mutant. Following cell stimulation, the protein-DNA complexes were immunoprecipitated with an anti-FLAG antibody. Enrichment of the CXCL11 (A), CXCL10 (B), and CCL2 (C) promoters by the IP was quantified by real-time PCR. *, p < 0.05; ***, p < 0.005; error bars represent S.E. (n = 3).