Table S1.

Primers used in this study

Primers for detecting KRAS mutation
Target	Forward primer (5′>>3′)	Reverse primer (5′>>3′)
KRAS exon 2–3	AGGCCTGCTGAAAATGACTG	TGGTGAATATCTTCAAATGATTTAGT
Primers for detecting EGFR mutation
Target	Forward primer (5′>>3′)	Reverse primer (5′>>3′)
EGFR exon 18–22	TGAAGGCTGTCCAACGAATG	AGGCGTTCTCCTTTCTCCAG
Primers for detecting HER2 mutation
Target	Forward primer (5′>>3′)	Reverse primer (5′>>3′)
HER2 exon 18–21	CCCTCTGACGTCCATCATCT	GCAGGGTCTGGACTGAAGAA
Primers for detecting BRAF mutation
Target	Forward primer (5′>>3′)	Reverse primer (5′>>3′)
BRAF exon 11–15	TCAGAAGACAGGAATCGAATGA	GATGACTTCTGGTGCCATCC