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. 2016 Jan 27;13(3):2492–2498. doi: 10.3892/mmr.2016.4814

Figure 4.

Figure 4

Runx2 affected BMP9-induced differentiation of C2C12 cells. (A) Ad-siRunx2 was used to knockdown the expression of Runx2. n=3, **P<0.01. (B-D) Co-infected Ad-siRunx2 and Ad-BMP9 cells were collected at 3 days. (B) ALP, (C) OCN and (D) OPN expression levels were detected by reverse transcription-quantitative polymerase chain reaction, n=3, *P<0.05 vs. the blank group. (E) ALP staining of BMP9-induced C2C12 myoblasts at day 7 of osteogenic differentiation treated with Ad-siRunx2 followed by transfection with mimics and inhibitors (magnification, ×200). **P<0.01 vs. BMP9 group. Runx2, runt-related transcription factor 2; BMP9, bone morphogenetic protein 9; Ad, adenovirus; si, short interfering; ALP, alkaline phosphatase; OCN, osteocalcin; OPN, osteopontin.