Table I.
Primer sequences of genes.
| Gene | Primer sequence (5′-3′) |
|---|---|
| Runx2 | F: GGTGAAACTCTTGCCTCGTC |
| R: AGTCCCAACTTCCTGTGCT | |
| ALP | F: TGCCTACTTGTGTGGCGTGAA |
| R: TCACCCGAGTGGTAGTCACAATG | |
| OCN | F: TCTGACAAAGCCTTCATGTCC |
| R: AAATAGTGATACCGTAGATGCG | |
| OPN | F: ACACTTTCACTCCAATCGTCC |
| R: TGCCCTTTCCGTTGTTGTCC | |
| miR-23b | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAATCA |
| F: GAGGGTTCCTGGCATGC | |
| R: GTGCAGGGTCCGAGGT | |
| GAPDH | F: AATGTGTCCGTCGTGGATCTGA |
| R: AGTGTAGCCCAAGATGCCCTTC | |
| U6 | RT: AAAATATGGAACGCTTCACGAATTTG |
| F: CTCGCTTCGGCAGCACATATACT | |
| R: ACGCTTCACGAATTTGCGTGTC |
Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; OCN, osteocalcin; OPN, osteopontin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.