Figure 2.

Immortalizing gene expression in SG-2, -3, -5, and -6 cells. (A) SV40 large T antigen immunofluorescence imaging with Alexa Fluor 594 and DAPI (nuclei). (B) Telomerase activity assessed by stretch polymerase chain reaction assay using 10–20% polyacrylamide gels and visualization with ethidium bromide, and are representative of three independent experiments. (C) Population doubling under hypoxic conditions. Population doubling and the incubation day were considered zero when single-cell cloning was performed. P/N-ctrl, positive/negative control.