Table I.
Nucleotide sequence of the primers used in PCR amplification.
| Gene | Primer sequence (5′-3′) |
|---|---|
| β-actin | F: GATTACTGCTCTGGCTCCTAGC |
| R: GACTCATCGTACTCCTGCTTGC | |
| IL-6 | F: CACAAGTCCGGAGAGGAGAC |
| R: CAGAATTGCCATTGCACAAC | |
| IL-1β | F: CGCAGCAGCACATCAACAAGAGC |
| R: TGTCCTCATCCTGGAAGGTCCACG | |
| MIP-2 | F: TGTCAATGCCTGAAGACCCTGCC |
| R: AACTTTTTGACCGCCCTTGAGAGTGG | |
| TNF-α | F: CACAGAAAGCATGATCCGCGAC |
| R: TGCCACAAGCAGGAATGAGAAGAG | |
| mCRAMP | F: AGCTACAGGGATGCTGTGCT |
| R: TCACTCGGAACCTCAGACT | |
| mBD1 | F: GGCATTCTCACAAGTCTTGGACGAAG |
| R: AGCTCTTACAACAGTTGGGCTTATCTGG |
F, forward; R, reverse; IL, interleukin; MIP, macrophage inflammatory protein; TNF, tumor necrosis factor; mCRAMP, mouse cathelicidin-related antimicrobial peptide; mBD1, mouse β-defensin 1.