Figure 1.

NC inhibits the proliferation of HCT116 cells. (A) Chemical structure of NC. (B) Following treatment with NC for 24 h (0, 2.5, 5, 10 and 20 µM), an MTT assay was used to measure the proliferation of the HCT116 cells. (C) Following treatment with 0, 10 and 20 µM NC for different time durations (0, 6, 12, 24 and 48 h), an MTT assay was used to detect the proliferation of the HCT116 cells. (D) Following treatment with NC for 40 h at different concentrations (0, 2.5, 5, 10 and 20 µM), a ³H-thymidine uptake assay was used to detect the proliferation of the HCT116 cells. The results are presented as the mean ± standard deviation from three independent experiments. ^*P<0.05, ^**P<0.01 vs. the control group; ^#P<0.05, ^##P<0.01 vs. the cells treated with 10 and 20 µM of NC. NC, nitidine chloride; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; cpm, counts per minute.