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NC increases the expression Bax, cleaved caspase-3 and -9 and reduces the expression of Bcl-2. (A) The HCT116 cells were treated with NC (0, 10 and 20 µM) for 24 h, and Bax, Bcl-2, cleaved caspase-3 and -9 expression levels in cell lysates were analyzed by western blotting. (B) Quantification of the western blotting results. Data are presented as the mean ± standard deviation from three independent experiments. **P<0.01 vs. the control group. NC, nitidine chloride.
