Figure 2.

miR-29c inhibition suppresses P19 cell apoptosis. Apoptosis was detected using flow cytometry in three independent experiments. Representative results are shown in the (A) (a) miR-29c inhibition group and (b) control group. (B) Apoptotic cell frequency is shown, and the magnitude of the inhibition of apoptosis by miR-29c was significant, comparison with that of the control group. Hoechst staining was performed to evaluate apoptosis in terms of cell morphology. (C) Following serum starvation, apoptosis of the cells began and the apoptotic rate was lower in the miR-29c inhibition group, compared with the control group (magnification, ×200). Bcl-2 gene family members were detected using (D) reverse transcription-quantitative polymerase chain reaction and (E) Western blot analyses. The results showed that the expression levels of survival-promoting Bcl-2 were significantly higher in the miR-29c inhibited group. Protein expression levels of cell death-promoting Bax were not affected (^*P<0.05). miR, microRNA; Bcl-2, B cell lymphoma 2; Bax, Bcl-2-associated X protein; Quad, quadrant.