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. 2016 Jan 29;13(3):2527–2535. doi: 10.3892/mmr.2016.4832

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Inhibition of miR-29c-suppresses P19 cell differentiation. (A) Comparison of cell morphology at day 4 between the (a) miR-29c-inhibited and (b) control group, and at day 10 in the (c) miR-29c-inhibited and (d) control group. (B) Characteristics of beating P19 cell clusters between days 10 and 14. (C) Expression levels of cardiogenesis-associated genes, determined using reverse transcription-quantitative polymerase chain reaction analysis (^*P<0.05 and ^**P<0.01). (D) Expression levels of cardiogenesis-associated proteins, determined using Western blot analysis. miR, microRNA; Bcl-2, B cell lymphoma-2; Bax, Bcl-2-associated X protein; Gata4, GATA binding protein 4; cTnT, cardiac troponin T; Mef2c, myocyte enhancer factor 2c; Wnt4, Wingless-related MMTV integration site 4.