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. 2016 Feb 26;11(2):e0149600. doi: 10.1371/journal.pone.0149600

Fig 4. TAM activation profile is altered by CDDO-Me.

Fig 4

F4/80+ tumor associated macrophages (TAMs) were isolated from the tumors of 12-week old female PyMT mice and treated in vitro with CDDO-Me (300 nM) for 16 hrs followed by stimulation with LPS (10 ng/ml) for an additional 24 hrs. Cell supernatants were analyzed by (A) ELISA to measure secreted protein levels. (B) Total RNA was isolated from TAMs +/- CDDO-Me. mRNA transcripts levels were measured by Taqman real time PCR. Multiplex analysis was used to quantify expression of cytokines and growth factors (C) and chemokines (D) in CDDO-Me-treated TAM supernatants. *p<0.05 vs. untreated control. (A-D) Data are representative of 3 independent experiments, and 3 technical replicates were analyzed in each experiment.