Table 3. Tajima’s relative rate test.

The equality of evolutionary rate analysis between sequences A (OsMPK6) and B (ScMPK-A), with sequence C (CjMPK) were used for the analysis of fungal MAPKs with a few AtMAPKs and OsMAPKs as the representative of T-E-Y and T-D-Y motifs. Sequences A (DeMPK) and B (AtMPK1), with sequence C (CjMPK) were used for fungal MAPKs with all the representative AtMAPKs and OsMAPKs as per default selection on the MEGA program. In the case of all fungal MAPKs that contain the novel activation loop motif, the equality of the evolutionary rate was calculated between sequences A (687684) and B (91669), with sequence C (5240). A P-value less than 0.05 is often used to reject the null hypothesis of equal rates between lineages. The analysis involved 3 amino acid sequences. All positions containing gaps and missing datas were eliminated. Evolutionary analyses were conducted in MEGA5.

Configuration	Fungal MAPKs having novel activation loop motifs with few AtMAPKs and OsMAPKs	Fungal MAPKs having novel activation loop motifs with all AtMAPKs and OsMAPKs	All Fungal MAPKs of that contain novel activation loop motif
Identical sites in all three sequences	130	117	193
Divergent sites in all three sequences	83	93	4
Unique differences in Sequence A	64	52	137
Unique differences in Sequence B	38	34	15
Unique differences in Sequence C	20	38	1
P-value	0.01004	0.0522	0.0000
X2 test	6.63	3.77	97.92
Degree of freedom	1	1	1