Table 4. Tajima’s test for neutrality.

Statistical analysis was carried out using MEGA5. In the statistical analysis, all the positions with site coverage 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. All ambiguous positions were removed for each sequence pair. Abbreviations: m = number of sequences; S = number of segregating sites; P_s = S/n; Θ = p_s/a_1; π = nucleotide diversity; and D is the Tajima test statistic.

Category	m	S	ps	Θ	π	D
All fungal MAPKs	1226	130	0.992366	0.129075	0.290760	3.500934
Only novel activation loop motif fungal MAPKs	24	265	0.880399	0.235761	0.514011	4.751476
Novel motif with two MAPKs of rice and Arabidopsis	30	280	0.906149	0.228730	0.534338	5.189926
Novel activation loop motif fungal MAPKs with all AtMAPKs and OsMAPKs	63	290	0.929487	0.197243	0.489426	5.233833