Table 5. Duplication analysis of novel activation loop motif of fungal MAPKs.

The most common activation loop motifs T-E-Y, T-G-Y, and K-G-Y of fungal MAPKs have Z-score of 10.86 with 100% confidence level, and are highly duplicated MAPKs. The MAPKs that contain the activation loop motifs T-T-Y, T-I-Y, S-E-Y, T-H-Y and T-N-Y, have a Z-score below four. Genes with Z-score of four or less were not considered as significant enough to be duplicated. Hence, MAPKs that contain Z-score below four are non duplicated MPAKs.

MAPK Genes with Activation loop Motif	Z-score	Level of confidence(%)
T-E-Y	10.86	100
T-G-Y	10.86	100
K-G-Y	10.86	100
T-Q-Y	8.64	100
S-D-Y	7.40	100
T-S-Y	5.99	100
T-T-Y	2.03	95.7
T-I-Y	1.99	95.3
S-E-Y	1.67	90.6
T-H-Y	1.49	86.4
T-N-Y	1.15	75