Skip to main content
. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: J Struct Biol. 2016 Feb 2;194(1):78–89. doi: 10.1016/j.jsb.2016.02.001

Table 1.

Comparison of Fc:FcγR affinities

IgG Variant Detection Method FcγRIIIa KD (nM) Variant Fold improved affinity compared with wtFc FcγRIIa KD (nM) Variant Fold improved affinity compared with wtFc FcγRIIb KD (nM) Fold improved affinity compared with wtFc Reference
wtFc (IgG1) Conventional SPR (FcR surface) 2950 F158 1380 H131 2520 Smith et al., 2012
Conventional SPR (FcR surface) 432 V158 1550 R131 Smith et al., 2012
Conventional SPR (FcR surface) 2220 F158 1330 R131 2700 Bournazos et al., 2014a
Conventional SPR (FcR surface) 2200 F158 1400 H131 2400 Bournazos et al., 2014b
Conventional SPR (FcR surface) 410 V158 1500 R131 Bournazos et al., 2014b
Conventional SPR (FcR surface) 2950 F158 1550 R131 2520 DiLillo et al., 2015
? 2000 ? 500 ? 500 Janeway et al., 2005
Conventional SPR (FcR surface) 854 F158 192 H131 8333 Bruhns et al., 2009
Conventional SPR (FcR surface) 500 V158 286 R131 Bruhns et al., 2009
Competition SPR (FcR surface) 252 V158 Lazar et al., 2006
Conventional SPR (FcR surface) 4454 F158 4486 H131 9275 current publication
SDALIE Fc Conventional SPR (FcR surface) 201 F158 22 1487 H131 3 2193 4 current publication
Competition SPR (FcR surface) – F158 318 – H131 2 – 5 current publication
GASDALIE Fc Conventional SPR (FcR surface) 100 F158 30 82 H131 17 736 3 Smith et al., 2012
124 V158 3 63 R131 25 Smith et al., 2012
Conventional SPR (FcR surface) 144 F158 15 104 R131 13 1670 2 Bournazos et al., 2014a
Conventional SPR (FcR surface) 110 F158 20 82 H131 17 1500 2 Bournazos et al., 2014b
Conventional SPR (FcR surface) 100 V158 4 120 R131 13 Bournazos et al., 2014b
Conventional SPR (FcR surface) 100 F158 30 63 R131 25 736 3 DiLillo et al., 2015
Conventional SPR (FcR surface) 213 F158 21 1585 H131 3 3882 2 current publication
Competition SPR (FcR surface) – F158 431 – H131 17 – 6 current publication