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. 2016 Feb 26;11(2):e0150539. doi: 10.1371/journal.pone.0150539

Fig 3. Characterization of transgenic Arabidopsis lines producing DXR-GFP.

Fig 3

(A) Relative levels of DXR transcripts in 30-day-old soil-grown wild type plants (C, white column) and 35S:DXR-GFP lines (grey and black columns) (n = 6 per group). The box on the right shows images of merged chlorophyll and GFP fluorescence signals in chloroplasts from the guard cells of lines representative of low (L), medium (M) and high (H) transgene expression levels (black columns). (B) Immunoblot analysis of DXR-GFP levels with an anti-GFP antibody in protein extracts (10 μg) from 10-day-old seedlings of the indicated lines. (C) Representative pictures of seedlings of the indicated lines germinated and grown for 10 days in the presence of fosmidomycin (50 μM). (D) Quantification of the phenotype observed in (C) as the percentage of seedling establishment (SE) and chlorophyll content (CHL) in the presence of fosmidomycin relative to those in the absence of inhibitor.