Fig 2. Specific binding of Hst 5, P113, hybrid peptides and di-18Hc to laminarin.

Binding/radial diffusion assay was performed by mixing various amounts of Candida cell wall components (laminarin, mannan and pustulan) with test peptides (final concentration of 180 μg/ml). 5 μl of each mixed samples were loaded into the well in the agar plate and 5 μl of each peptide solutions (180 μg/ml) was used for the polysaccharides-free control. The anti-Candida activities of peptides in the mixture were graphed against the concentration of polysaccharides. Diameters of the clearing zone are expressed in units (1 mm = 10 U).