Fig 7. The effect of peptides on ROS generation.

Time-dependency of DCF fluorescence as a measure of ROS generation (A) and killing activity (B) were determined in Candida cells treated with peptides (32 μg/ml) or peptides + Ascorbic acid (200 μg/ml) by Perkin Elmer 2030 reader (VictorX 3) and colony count assay, respectively. The fluorescence obtained with the cells treated with 10 mM of H₂O₂ served as positive control and the cells without peptide served as negative control. The fluorescent intensity of DCF was recorded every 10 min for 120 min at excitation and emission wavelengths of 485 and 530 nm, respectively.