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. 2016 Mar;24(3):544–554. doi: 10.1016/j.joca.2015.10.003

Fig. 1.

Fig. 1

IFT88 mutation increases F-actin and non-muscle myosin IIB expression independent of RhoA. (A) Representative confocal maximum intensity Z projections showing F-actin and myosin IIB immuno-fluorescently labelled for WT and IFT88orpk cells in sub-confluent cultures. F-actin was stained with Alexa Fluor 555-phalloidin (red) while myosin IIB was immuno-fluorescently labelled for NMIIB (green). Scale bar represents 25 μm. (B) Quantification of F-actin and myosin IIB for WT and IFT88orpk cells in sub-confluent cultures. Fluorescence intensities were quantified from epifluorescence images and normalised to the mean intensity in WT cells. Data represents mean ± CI (N = 32 and 36). Data was analysed by unpaired Student's t test. (C) RhoA activity for WT and IFT88orpk cells in sub-confluent cultures. RhoA activity was measured using a RhoA G-LISA assay. Data represents mean ± CI (N = 9 and 10). Data was analysed by unpaired Student's t test.