Fig. 2.

IFT88 mutation increases disassembly of F-actin associated with increased myosin IIB expression with cytochalasin D treatment. (A) Representative epifluorescence images showing the changes of F-actin (top panels) and myosin IIB (bottom panels) in WT and IFT88^orpk cells exposed to cytochalasin D (6 μM) for 10 min. F-actin was stained with Alexa Fluor 555-phalloidin while myosin IIB was labelled with antibodies targeted to NMIIB. Cell edges were identified by the red lines drawn around individual cells. Scale bar represents 25 μm. Changes in mean fluorescence intensity of (B) F-actin and (C) myosin IIB for WT and IFT88^orpk cells under each condition. Fluorescence intensity was normalized to the mean intensity of corresponding untreated WT cell groups. Data represents mean ± CI (N = 32 and 36). Data was analysed by unpaired Student's t test.