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. 2016 Mar;24(3):544–554. doi: 10.1016/j.joca.2015.10.003

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 6 — IFT88^orpkcells exhibit decreased cortical tension and increased deformability of F-actin cortex. (A) IFT88^orpk cells exhibit decreased cortical tension compared with WT cells. Data represents median and quartiles (N = 20 and 17). Data was analysed by Mann–Whitney U test. (B) IFT88^orpk cells exhibit a significant increase in aspiration length before bleb formation. Data represents median and quartiles (N = 27 and 36). Data was analysed by Mann–Whitney U test. (C) Representative confocal images showing a LifeAct-GFP tagged cell exhibiting no blebs during aspiration. White arrows indicate the leading edge of cortical F-actin and black arrows for the leading edge of cell membrane. (D) Instantaneous modulus and (E) equilibrium modulus for cells exhibiting no blebs were estimated using SLS model. Data represents median and quartiles (N = 10 and 21). Data was analysed by Mann–Whitney U test.