Table 1.
Relevant stimulatory peptides mapped in cultured IFN-γ ELISPOT assay in HIV-CORE 002 (modified from Ref. [22]).
| Peptide |
Volunteer no. |
||||
|---|---|---|---|---|---|
| No. | HXB2 aa position | Sequence | 411 | 418 | 421 |
| 3 | Gag 166-171 | EVIPMFTALSEGATP | – | 1200a | – |
| 17 | Gag 268-282 | GLNKIVRMYSPVSIL | – | 1583 | 517 |
| 31 | Gag 426-440 | CTERQANFLGKIWPS | – | – | 683 |
| 47 | Pol 154-168 | LTQIGCTLNFPISPI | – | 600 | – |
| 49 | Pol 165-179 | KNFPISPIETVPVKLKb | – | 883 | – |
| 67 | Pol 226/227-240 | W-RKLVDFRELNKRTQc | – | – | 483 |
| 78 | Pol 270-284 | YFSVPLDEGFRKYTA | – | – | 2367 |
| 80 | Pol 277-293 | GFRKYTAFTIPSINN | 1917 | 667 | – |
| 88 | Pol 311-325 | GSPAIFQSSMTKILE | 750 | – | 2017 |
| 90 | Pol 319-333 | SMTKILEPFRAQNPE | – | – | – |
| 93 | Pol 331-345 | KNPEIVIYQYMDDLYVb | 3967 | 4133 | 650 |
| 94 | Pol 335-349 | VIYQYMDDLYVGSDL | 750 | 1300 | – |
| 116 | Pol 563-577 | ATWIPEWEFVNTPPL | 617 | – | – |
| 117 | Pol 567-581 | PEWEFVNTPPLVKLW | 1283 | – | – |
| 135 | Pol 706-720 | KLVSQGIRKVLFLDG | – | 2050 | – |
| 164 | Pol 891-906 | VQMAVFIHNFKRKGGI | – | – | 517 |
| 175 | Pol 970-984 | SDIKVVPRRKAKIIR | – | – | 700 |
a
Numbers indicate cultured IFN-γ ELISPOT assay frequencies as SFU/106 PBMCs [22].
b
Underlined K indicates lysine added to improve peptide solubility.
c
‘–’ dash in peptide sequence indicates junction between adjacent conserved regions.